|
| Status |
Public on Mar 30, 2015 |
| Title |
Untransfected cells 0h rep 3 (SETX reanalysis) |
| Sample type |
RNA |
| |
|
| Source name |
A549 cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: no siRNA
infection: no infection
cell line: A549
|
| Treatment protocol |
A549 cells were transfected with either control siRNA (D-001810-10-05, Dharmacon) or SETX siRNA (23064, Dharmacon) pools using the Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen). Transfections were performed according to the manufacturer’s instructions and cells were used for further experiments 48 hours post intransfection.RNA and protein levels of SETX were quantified to determine knockdown efficiency.
|
| Growth protocol |
Standard culture techniques
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN Rneasy mini kit
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled RNA was prepared with MessageAmp™ Premier RNA Amplification Kit (Applied Biosystems)
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
Replicate 3
|
| Data processing |
Data was analysed using Genespring GX12.5 . To analyze gene expression changes during stimulation, raw data were subject to quantile normalization and baseline transformation was performed to the median of all 9 samples
|
| |
|
| Submission date |
Dec 03, 2013 |
| Last update date |
Mar 30, 2015 |
| Contact name |
Ivan Marazzi |
| Organization name |
Icahn School of Medicine at Mount Sinai
|
| Department |
Department of Microbiology
|
| Street address |
1468 Madison Avenue
|
| City |
New York |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE52933 |
Analysis of global gene expression profiles of A549 cells treated with SETX siRNA under basal conditions |
| GSE52937 |
Senataxin suppresses the antiviral transcriptional response and controls viral biogenesis |
|
| Relations |
| Reanalysis of |
GSM1278305 |
