|
| Status |
Public on Dec 01, 2014 |
| Title |
linc00467 siRNA transfected |
| Sample type |
RNA |
| |
|
| Source name |
neuroblasotma BE(2)-C cells transfected with linc00467 siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral nervous system tumour cell
genotype: MYCN-amplified neuroblastoma cells
treatment: linc00467 siRNA
cell line: BE(2)-C
|
| Treatment protocol |
BE(2)-C cells were transfected with control siRNA or linc00467 siRNA for 48 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome HuGene-2_0-st Array. GeneChips were washed and stained.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray scanner.
|
| Description |
Gene expression data from neuroblastoma cells knocking-down of lncMycN
BE(2)-C neuroblastoma cells were cultured in Roswell Park Memorial Institute Medium (RPMI) 1640 supplemented with 10% fetal calf serum and 1% L-glutamine.
|
| Data processing |
Images were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
Results were further analysed in R (http://www.r-project.org/) with bioconductor package (http://www.bioconductor.org/).
|
| |
|
| Submission date |
Dec 04, 2013 |
| Last update date |
Dec 01, 2014 |
| Contact name |
Tao Liu |
| E-mail(s) |
tliu@ccia.unsw.edu.au
|
| Phone |
61293851935
|
| Organization name |
Children's Cancer Institute Australia
|
| Street address |
Lowy Cancer Research Centre UNSW
|
| City |
Randwick, Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2031 |
| Country |
Australia |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE52985 |
Differential gene expression in neuroblastoma cells after transfection with control siRNA or linc00467 siRNA |
|
