|
| Status |
Public on Dec 11, 2015 |
| Title |
SilX-BAHD1.R1 |
| Sample type |
RNA |
| |
|
| Source name |
SilX-BAHD1 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293
genotype/variation: BAHD1 knockdown
|
| Treatment protocol |
We established a BAHD1 gene-specific knockdown cell line using the SilenciX® siRNA delivery system (Tebu-bio). This technology is based on EBV-replication, and ensures chromosome-like replication of an episome stably expressing either a shRNA against BAHD1 (silXBAHD1) or a control shRNA (silX-CT).
|
| Growth protocol |
Cells were grown at 37°C, in a humidified 10% CO2 incubator, in Dulbecco's modified Eagle's medium with GlutaMAX (Gibco) supplied with 10%FBS (Gibco) and 70 ug/ml Hygromycin B (10687-010-Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen), from three biological replicates, according to the manufacturer's instructions. RNA was treated with DNase I to remove residual DNA and then cleaned with DNase Inactivation Reagent (Ambion® Inc.) RNA was quality checked an then store at -80°C.
|
| Label |
biotin
|
| Label protocol |
RNA was amplified with GeneChip® WT cDNA Synthesis and Amplification Kit (Affymetrix) and biotin labeld with GeneChip® WT Terminal Labeling Kit (Affymetrix) according to the manufacturer's
|
| |
|
| Hybridization protocol |
Labeled cDNA was hybridized wiht GeneChip® Hybridization, Wash, and Stain Kit (Affymetrix) as described by the manufacturer.
|
| Scan protocol |
Affymetrix Human Gene 1.0 ST Array was scanned with the Affymetrix GeneChip Scanner 7G and the FS450_0007 protocol , as recommended in the GeneChip® Hybridization, Wash, and Stain Kit (Affymetrix)
|
| Description |
Gene expression data
|
| Data processing |
Data were normalized with RMA
|
| |
|
| Submission date |
Dec 13, 2013 |
| Last update date |
Jan 20, 2016 |
| Contact name |
Helene Bierne |
| E-mail(s) |
helene.bierne@inrae.fr
|
| Phone |
33134652289
|
| Organization name |
INRAE - University PARIS SACLAY
|
| Department |
Micalis Institute
|
| Lab |
Epigenetics and Cellular Microbiology
|
| Street address |
Domaine de Vilvert, bat 442, INRA Micalis
|
| City |
Jouy-en-Josas |
| ZIP/Postal code |
78350 |
| Country |
France |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE51868 |
HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1) |
| GSE53284 |
Transcriptome of HEK293 cells stably knockdown for the BAHD1 gene with a shRNA |
|
