|
Status |
Public on Dec 11, 2015 |
Title |
SilX-BAHD1.R3 |
Sample type |
RNA |
|
|
Source name |
SilX-BAHD1 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: HEK293 genotype/variation: BAHD1 knockdown
|
Treatment protocol |
We established a BAHD1 gene-specific knockdown cell line using the SilenciX® siRNA delivery system (Tebu-bio). This technology is based on EBV-replication, and ensures chromosome-like replication of an episome stably expressing either a shRNA against BAHD1 (silXBAHD1) or a control shRNA (silX-CT).
|
Growth protocol |
Cells were grown at 37°C, in a humidified 10% CO2 incubator, in Dulbecco's modified Eagle's medium with GlutaMAX (Gibco) supplied with 10%FBS (Gibco) and 70 ug/ml Hygromycin B (10687-010-Invitrogen).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen), from three biological replicates, according to the manufacturer's instructions. RNA was treated with DNase I to remove residual DNA and then cleaned with DNase Inactivation Reagent (Ambion® Inc.) RNA was quality checked an then store at -80°C.
|
Label |
biotin
|
Label protocol |
RNA was amplified with GeneChip® WT cDNA Synthesis and Amplification Kit (Affymetrix) and biotin labeld with GeneChip® WT Terminal Labeling Kit (Affymetrix) according to the manufacturer's
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Hybridization protocol |
Labeled cDNA was hybridized wiht GeneChip® Hybridization, Wash, and Stain Kit (Affymetrix) as described by the manufacturer.
|
Scan protocol |
Affymetrix Human Gene 1.0 ST Array was scanned with the Affymetrix GeneChip Scanner 7G and the FS450_0007 protocol , as recommended in the GeneChip® Hybridization, Wash, and Stain Kit (Affymetrix)
|
Description |
Gene expression data
|
Data processing |
Data were normalized with RMA
|
|
|
Submission date |
Dec 13, 2013 |
Last update date |
Jan 20, 2016 |
Contact name |
Helene Bierne |
E-mail(s) |
helene.bierne@inrae.fr
|
Phone |
33134652289
|
Organization name |
INRAE - University PARIS SACLAY
|
Department |
Micalis Institute
|
Lab |
Epigenetics and Cellular Microbiology
|
Street address |
Domaine de Vilvert, bat 442, INRA Micalis
|
City |
Jouy-en-Josas |
ZIP/Postal code |
78350 |
Country |
France |
|
|
Platform ID |
GPL6244 |
Series (2) |
GSE51868 |
HEK293 cells (HEK293-CT) and HEK293 cells stably over-expressing the BAHD1 gene (HEK-BAHD1) |
GSE53284 |
Transcriptome of HEK293 cells stably knockdown for the BAHD1 gene with a shRNA |
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