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Sample GSM1300288 Query DataSets for GSM1300288
Status Public on Jan 15, 2015
Title zero-hour biopsy 1073, biopsy pair 1073-07, AKI group
Sample type RNA
 
Source name kidney allograft biopsy
Organism Homo sapiens
Characteristics tissue: kidney
biopsy type: zero-hour biopsy
Extracted molecule total RNA
Extraction protocol Kidney tissue in RNAlater, stored at -80°C. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA (200 ng per sample) was amplified and labeled using the Applause WT-Amp ST and Encore Biotin Module kit (NuGEN, San Carlos, CA)
 
Hybridization protocol cDNA were hybridized for 18 hr at 45C on GeneChip Human ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Arrays were scanned on an Affymetrix GeneChip scanner 7G.
Description Gene expression data from zero-hour biopsy
Data processing Normalized expression signals were calculated from Affymetrix CEL files using RMA (Irizarry et al., 2003).
 
Submission date Jan 02, 2014
Last update date Jan 15, 2015
Contact name Julia Wilflingseder
E-mail(s) julia.wilflingseder@meduniwien.ac.at
Organization name Medical University of Vienna
Department Nephrology and Dialysis
Lab Laboratory Rainer Oberbauer
Street address Währinger Gürtel 18-20
City Vienna
ZIP/Postal code 1090
Country Austria
 
Platform ID GPL16686
Series (2)
GSE53769 Molecular regulation of acute kidney injury (mRNA)
GSE53773 Molecular regulation of acute kidney injury

Data table header descriptions
ID_REF
VALUE log2 intensity (RMA)

Data table
ID_REF VALUE
16650001 5.011668
16650003 3.309931
16650005 5.289571
16650007 2.492674
16650009 1.930611
16650011 4.753288
16650013 2.776595
16650015 2.886436
16650017 2.547703
16650019 3.182786
16650021 3.687388
16650023 4.201232
16650025 3.753766
16650027 2.011036
16650029 3.777299
16650031 5.306172
16650033 2.487779
16650035 1.800067
16650037 2.738923
16650041 5.437021

Total number of rows: 53617

Table truncated, full table size 938 Kbytes.




Supplementary file Size Download File type/resource
GSM1300288_1073_ATN_7.CEL.gz 7.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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