|
| Status |
Public on Jul 20, 2014 |
| Title |
V8_05_JL |
| Sample type |
RNA |
| |
|
| Source name |
Vaginal Epithelium
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Vaginal Epithelium
visit: 8
first intervention: probiotic
second intervention: placebo
responder status: nonresponder
ph: 6.5
nugent score: 3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples collected and stored in 500 µL RNAlater and frozen at -80˚C until extraction. Samples were thawed on ice, vortexed, and centrifuged (5,000 g, 10 min, 4˚C). The supernatant was discarded and the RNA was extracted using TRIzol and Turbo DNase (Life Technologies) according to the manufacturers protocols.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
RMA
|
| |
|
| Submission date |
Jan 23, 2014 |
| Last update date |
Jul 20, 2014 |
| Contact name |
Jordan Eduard Bisanz |
| E-mail(s) |
jbisanz@uwo.ca
|
| Organization name |
Lawson Health Research Institute
|
| Street address |
268 Grosvenor Street, Room F2-116
|
| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A 4V2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE54363 |
Effect of L. rhamnosus GR-1 and L. reuteri RC-14 on host responses in a trial of post-menopausal women |
|
