|
Status |
Public on Jul 20, 2014 |
Title |
V4_07_AR |
Sample type |
RNA |
|
|
Source name |
Vaginal Epithelium
|
Organism |
Homo sapiens |
Characteristics |
tissue: Vaginal Epithelium visit: 4 first intervention: placebo second intervention: probiotic responder status: nonresponder ph: 5 nugent score: 4
|
Extracted molecule |
total RNA |
Extraction protocol |
Samples collected and stored in 500 µL RNAlater and frozen at -80˚C until extraction. Samples were thawed on ice, vortexed, and centrifuged (5,000 g, 10 min, 4˚C). The supernatant was discarded and the RNA was extracted using TRIzol and Turbo DNase (Life Technologies) according to the manufacturers protocols.
|
Label |
biotin
|
Label protocol |
standard Affymetrix protocol
|
|
|
Hybridization protocol |
standard Affymetrix protocol
|
Scan protocol |
standard Affymetrix protocol
|
Data processing |
RMA
|
|
|
Submission date |
Jan 23, 2014 |
Last update date |
Jul 20, 2014 |
Contact name |
Jordan Eduard Bisanz |
E-mail(s) |
jbisanz@uwo.ca
|
Organization name |
Lawson Health Research Institute
|
Street address |
268 Grosvenor Street, Room F2-116
|
City |
London |
State/province |
Ontario |
ZIP/Postal code |
N6A 4V2 |
Country |
Canada |
|
|
Platform ID |
GPL16686 |
Series (1) |
GSE54363 |
Effect of L. rhamnosus GR-1 and L. reuteri RC-14 on host responses in a trial of post-menopausal women |
|