|
| Status |
Public on May 26, 2015 |
| Title |
2xNEG cells, derived from the EpCAM positive population whithin the xenograft |
| Sample type |
genomic |
| |
|
| Source name |
patient derived xenograft
|
| Organism |
Homo sapiens |
| Characteristics |
xenograft model: mas9806
sub-population: 2xNEG
|
| Treatment protocol |
Single cell suspension from an luminal-like breast cancer was prepared by enzymatic disaggregation of tumours. The cells were re-suspended in cold staining buffer (PBS containing 0.5 % FCS and 3 % human immune globulin (Gammaguard) (N.V Baxter S.A, Belgium) and stained with fluorescently-labelled antibodies, diluted according to the manufacturers’s recommendation. Following 30 min incubation at 4 oC, the stained cells were washed ones with PBS, then re-suspended in DMEM/F12 with 0.,5% human serum albumin, 2% Hepes, Penicillin and Streptomycin and further sorted by FACS DIVA flow cytometer (Becton Dickinson), equipped with a 488nm Argon laser (Coherent) and 633nm HeNe laser (Spectra Physics).The single cell suspensions were stained with 1 µg/ml propidium iodide (PI) (Sigma) prior to sorting to exclude the dead cells from the analysis.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNAfrom FACS sorted cell subpopulations was isolated using Qiagen DNA extraction kit according to manufacturers protocol.
|
| Label |
c-bio; a-DNP
|
| Label protocol |
Standard Illumina Infinium HD protocol
|
| |
|
| Hybridization protocol |
Standard Illumina Infinium HD protocol
|
| Scan protocol |
Standard Illumina Infinium HD protocol
|
| Description |
genomic DNA extracted from sorted xenograft cells,was genotyped using Infinium HumanOmniexpressExome Genotyping BeadChips (Illumina)
|
| Data processing |
Intensity data were extracted in Genome studio (v2011.1)
|
| |
|
| Submission date |
Jan 31, 2014 |
| Last update date |
May 26, 2015 |
| Contact name |
Nirma Skrbo |
| E-mail(s) |
nirlar@rr-research.no
|
| Phone |
+4722781859
|
| Organization name |
Oslo University Hospital
|
| Department |
Dep of Genetics
|
| Lab |
Sørlie
|
| Street address |
Ullernchauseen 70
|
| City |
Oslo |
| ZIP/Postal code |
0310 |
| Country |
Norway |
| |
|
| Platform ID |
GPL16333 |
| Series (2) |
| GSE54595 |
Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer model (SNP) |
| GSE56103 |
Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer model |
|
