|
| Status |
Public on Feb 27, 2014 |
| Title |
MCF7 shCTL rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MCF7, shRNA Control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast cancer
cell line: MCF7
endocrine sensitivity: endocrine sensitive
|
| Treatment protocol |
Cells were transduced with shLRH-1 or shCTL lentiviruses for 4 days before mRNA extraction.
|
| Growth protocol |
MCF7 are growth in DMEM containing 10% fetal bovine serum (FBS), glutamine and antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the GenElute mammalian total RNA miniprep kit (Sigma).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to standard Illumina protocol at McGill University & Genome Quebec Innovation Centre
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Log2 transformation and quantile normalization of the data were performed with FlexArray 1.6.3 software
|
| |
|
| Submission date |
Feb 11, 2014 |
| Last update date |
Feb 27, 2014 |
| Contact name |
Nicolas Gévry |
| E-mail(s) |
nicolas.gevry@usherbrooke.ca
|
| Organization name |
Université de Sherbrooke
|
| Street address |
2500 boulevard de l'université
|
| City |
Sherbrooke |
| State/province |
Québec |
| ZIP/Postal code |
J1K 2R1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE54891 |
LRH-1 governs vital transcriptional programs in endocrine sensitive and resistant breast cancer cells: Expression profiling |
| GSE54892 |
LRH-1 governs vital transcriptional programs in endocrine sensitive and resistant breast cancer cells |
|
