|
| Status |
Public on Feb 13, 2014 |
| Title |
dendritic cell_sulforaphane treated_replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse dendritic cells, Solforaphane treated
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
treatment: Sulforaphane
cell type: dendritic cells
|
| Treatment protocol |
DC were harvested on day 7, incubated with 0.3 µM sulforaphane (Sigma-Aldrich) in DMSO or DMSO alone as control for 2 hours and then activated with 100 ng/ml LPS from E. coli O111:B4 (Sigma-Aldrich) for 1 hour.
|
| Growth protocol |
Bone marrow-derived DC from C57BL/6 mice were isolated and cultivated as described in Lutz MB et al., J Immunol Methods 1999;223:77-92.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
DC were lysed and total RNA was isolated using the peqGOLD Total RNA Kit (peqlab) following the manufacturer's protocols.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA using the GeneChip® WT PLUS Reagent Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix MoGene 2.1 ST Array Plates. Hybridization, washing, staining and scanning was performed fully automated on a Gene Titan instrument using GeneChip® Hybridization, Wash, and Stain Kit (Affymetrix).
|
| Scan protocol |
see hyb protocol
|
| Description |
I13R022d01
|
| Data processing |
The data were analysed using Bioconductor package 'oligo' 1.20.4 under R-2.15.1. Background correction, normalization and probe summarization was performed with RMA.
|
| |
|
| Submission date |
Feb 13, 2014 |
| Last update date |
Feb 13, 2014 |
| Contact name |
Michael H. Walter |
| E-mail(s) |
michael.walter@agilent.com
|
| Organization name |
University of Tuebingen
|
| Department |
Department of Medical Genetics
|
| Lab |
The Microarray Facility Tübingen
|
| Street address |
Calwer Str. 7
|
| City |
Tuebingen |
| ZIP/Postal code |
72076 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE54980 |
Sulforaphane protects from T cell mediated autoimmune disease by inhibition of interleukin 23 and 12 in dendritic cells |
|
