|
| Status |
Public on Jun 02, 2014 |
| Title |
DU145 FOXA1 |
| Sample type |
RNA |
| |
|
| Source name |
DU145
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DU145
genotype/variation: FOXA1 overexpression
|
| Treatment protocol |
Cells were collected at 48 hours after adenoviral overexpression of LacZ, AR, FOXA1 or AR+FOXA1 proteins.
|
| Growth protocol |
DU145 cells were maintained in RPMI supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. The quantity and quality of the isolated RNAs was determined using ND-1000 Spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| Scan protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| Description |
SAMPLE 3
|
| Data processing |
The bead-level data were preprocessed using GenomeStudio (Illumina) and the expression data was analyzed using the beadarray package in Bioconductor,quantile normalized using the beadarray package.
|
| |
|
| Submission date |
Feb 13, 2014 |
| Last update date |
Jun 02, 2014 |
| Contact name |
Hong-Jian Jin |
| E-mail(s) |
hongjian-jin@northwestern.edu
|
| Phone |
3125033041
|
| Organization name |
Northwestern University
|
| Department |
Medicine
|
| Street address |
303 E Superior St
|
| City |
Chicago |
| State/province |
IL - Illinois |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE54991 |
Cooperativity and Equilibrium with FOXA1 Define Androgen Receptor Transcriptional Program [array] |
| GSE55007 |
Cooperativity and Equilibrium with FOXA1 Define Androgen Receptor Transcriptional Program |
|
