NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1327525 Query DataSets for GSM1327525
Status Public on Jun 02, 2014
Title DU145 AR+FOXA1
Sample type RNA
 
Source name DU145
Organism Homo sapiens
Characteristics cell line: DU145
genotype/variation: AR+FOXA1 overexpression
Treatment protocol Cells were collected at 48 hours after adenoviral overexpression of LacZ, AR, FOXA1 or AR+FOXA1 proteins.
Growth protocol DU145 cells were maintained in RPMI supplemented with 10% FBS
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions. The quantity and quality of the isolated RNAs was determined using ND-1000 Spectrophotometer.
Label biotin
Label protocol The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
 
Hybridization protocol The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
Scan protocol The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
Description SAMPLE 4
Data processing The bead-level data were preprocessed using GenomeStudio (Illumina) and the expression data was analyzed using the beadarray package in Bioconductor,quantile normalized using the beadarray package.
 
Submission date Feb 13, 2014
Last update date Jun 02, 2014
Contact name Hong-Jian Jin
E-mail(s) hongjian-jin@northwestern.edu
Phone 3125033041
Organization name Northwestern University
Department Medicine
Street address 303 E Superior St
City Chicago
State/province IL - Illinois
ZIP/Postal code 60611
Country USA
 
Platform ID GPL10558
Series (2)
GSE54991 Cooperativity and Equilibrium with FOXA1 Define Androgen Receptor Transcriptional Program [array]
GSE55007 Cooperativity and Equilibrium with FOXA1 Define Androgen Receptor Transcriptional Program

Data table header descriptions
ID_REF
VALUE background-subtracted, quantile-normalized expression value
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 2.4 0.31039
ILMN_2055271 5.5 0.15195
ILMN_1736007 5.7 0.13896
ILMN_2383229 -3.5 0.73117
ILMN_1806310 2.4 0.30779
ILMN_1779670 -2.7 0.66364
ILMN_1653355 -2.8 0.67532
ILMN_1717783 -7.2 0.93506
ILMN_1705025 5.3 0.17143
ILMN_1814316 8.7 0.07273
ILMN_2359168 -2.1 0.62078
ILMN_1731507 -2.9 0.68182
ILMN_1787689 -5.6 0.87013
ILMN_3241953 98.5 0
ILMN_1745607 -5.8 0.88182
ILMN_2136495 -5.9 0.89221
ILMN_1668111 1.6 0.35065
ILMN_2295559 -7.1 0.93506
ILMN_1735045 20 0.00779
ILMN_1680754 12.5 0.03117

Total number of rows: 39991

Table truncated, full table size 931 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap