|
Status |
Public on Feb 15, 2014 |
Title |
Input_control |
Sample type |
SRA |
|
|
Source name |
CAL-1 plasmacytoid dendritic cells
|
Organism |
Homo sapiens |
Characteristics |
cell type: plasmacytoid dendritic cell chip antibody: preimmune rabbit IgG (Bethyl Labs P120-101)
|
Growth protocol |
The human CAL-1 plasmacytoid dendritic cell line was grown RPMI media supplemented with 10% FBS and 2mM L-Glutamine. Media was changed every 2-3 days.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E6200L ) following by manufacturer’s instructions.
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
Input
|
Data processing |
Illumina Casava1.7 software used for basecalling. Sequenced reads were mapped to mouse hg18 genome using BWA version 0.5.9-r16. Peaks were called using a Parzen window based algorithm (LEE et al, 2011. Nucleic Acis Res. 39(9):3558-73). Genome_build: hg18 Supplementary_files_format_and_content: tab-delimited text files include information regarding chromosome, peak start, peak end, peak score, and peak center for each peak
|
|
|
Submission date |
Feb 14, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Bum-Kyu Lee |
E-mail(s) |
bklee92@gmail.com
|
Phone |
512-232-8046
|
Organization name |
University of Texas at Austin
|
Street address |
2506 Speedway Stop A5000
|
City |
Austin |
State/province |
TX |
ZIP/Postal code |
78712 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE55043 |
Dendritic Cell Fate is Determined by BCL11A Â |
|
Relations |
BioSample |
SAMN02642567 |
SRA |
SRX471324 |