|
| Status |
Public on Mar 13, 2014 |
| Title |
Liver cancer xenograft, model ID 03-0005 |
| Sample type |
RNA |
| |
|
| Source name |
xenograft RNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: xenograft passage 3
tissue: xenograft liver tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Genomic RNA was isolated using RNeasy protect mini kit (Qiagen). The concentrations were quantified using NanoDrop ND-1000 spectrophotometer (NanoDrop, Wilmington, DE). RNA samples with an RNA integrity number above 8.0 and A260/280 ratios between 1.8 and 2.0 were used for gene expression array.
|
| Label |
biotin
|
| Label protocol |
Total RNA was amplified and fragmented using a GeneChip 3’ IVT expression kit (Affymetrix, Santa Clara, CA). Then the samples were hybridized onto a GeneChip PrimeView™ human gene expression array (Affymetrix).
|
| |
|
| Hybridization protocol |
Total RNA was amplified and fragmented using a GeneChip 3’ IVT expression kit (Affymetrix, Santa Clara, CA). Then the samples were hybridized onto a GeneChip PrimeView™ human gene expression array (Affymetrix).
|
| Scan protocol |
Scanned on an Affymetrix GeneChip scanner 3000 7G
|
| Data processing |
Processed on Affymetrix Expression Console version 1.1, with RMA normalization approach.
|
| |
|
| Submission date |
Mar 12, 2014 |
| Last update date |
Mar 13, 2014 |
| Contact name |
Douglas Fang |
| E-mail(s) |
douglas_fang@wuxiapptec.com
|
| Organization name |
WuXi AppTec Co., Ltd.
|
| Street address |
#288 FuTe Zhong Road, WaiGaoQiao Free Trade Zone
|
| City |
Shanghai |
| ZIP/Postal code |
200131 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE55828 |
Characterization of a large panel of patient-derived hepatocellular carcinoma xenograft tumor models for preclinical development |
|
