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Sample GSM1361812 Query DataSets for GSM1361812
Status Public on Aug 08, 2014
Title SEQC_RTPCR_A
Sample type RNA
 
Source name Universal Human Reference RNA (UHRR) from Stratagene
Organism Homo sapiens
Characteristics seqc sample: A
Extracted molecule total RNA
Extraction protocol none
Label SYBR Green
Label protocol PCR assay; total volume: 5 μl; instrument: CFX384 (with automation); mastermix: SsoAdvanced SYBR; primer concentration: 250 nM each; Samples: cDNA: 25 ng (total RNA equivalents); gDNA: 2.5 ng (Roche); NTC: water + carrier (5 ng/μl yeast transfer RNA); synthetic template (pooled 60-mers in concentration range: 2E7 – 2E1 copies); PCR program: default cycling protocol for SsoAdvanced SYBR (Ta=60°C)
 
Hybridization protocol n/a
Scan protocol n/a
Data processing non-normalized raw Cq data. With single molecule Cq = 35
 
Submission date Apr 02, 2014
Last update date Aug 08, 2014
Contact name Leming Shi
E-mail(s) lemingshi@fudan.edu.cn
Phone +86-18616827008
Organization name Fudan University
Department School of Life Sciences
Lab Center for Pharmacogenomics
Street address 2005 Songhu Road
City Shanghai
ZIP/Postal code 200438
Country China
 
Platform ID GPL18522
Series (2)
GSE47792 SEQC Project
GSE56457 A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequence Quality Control consortium

Data table header descriptions
ID_REF
VALUE raw Cq values. With single molecule Cq = 35

Data table
ID_REF VALUE
1 30.24
2 37.66
3 27.57
4 24.7
5 43.3
6 28.39
7 25.91
8 42.99
9 24.89
10 31.38
11 19.61
12 30.75
13 24.71
14 33.5
15 22.08
16 28.26
17 23.06
18 42.48
19 29.79
20 30.54

Total number of rows: 20801

Table truncated, full table size 228 Kbytes.




Supplementary data files not provided
Processed data not provided for this record
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