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Sample GSM1361815 Query DataSets for GSM1361815
Status Public on Aug 08, 2014
Title SEQC_RTPCR_D
Sample type RNA
 
Source name Mix of A and B (1:3)
Organism Homo sapiens
Characteristics seqc sample: D
Extracted molecule total RNA
Extraction protocol none
Label SYBR Green
Label protocol PCR assay; total volume: 5 μl; instrument: CFX384 (with automation); mastermix: SsoAdvanced SYBR; primer concentration: 250 nM each; Samples: cDNA: 25 ng (total RNA equivalents); gDNA: 2.5 ng (Roche); NTC: water + carrier (5 ng/μl yeast transfer RNA); synthetic template (pooled 60-mers in concentration range: 2E7 – 2E1 copies); PCR program: default cycling protocol for SsoAdvanced SYBR (Ta=60°C)
 
Hybridization protocol n/a
Scan protocol n/a
Data processing non-normalized raw Cq data. With single molecule Cq = 35
 
Submission date Apr 02, 2014
Last update date Aug 08, 2014
Contact name Leming Shi
E-mail(s) lemingshi@fudan.edu.cn
Phone +86-18616827008
Organization name Fudan University
Department School of Life Sciences
Lab Center for Pharmacogenomics
Street address 2005 Songhu Road
City Shanghai
ZIP/Postal code 200438
Country China
 
Platform ID GPL18522
Series (2)
GSE47792 SEQC Project
GSE56457 A comprehensive assessment of RNA-seq accuracy, reproducibility and information content by the Sequence Quality Control consortium

Data table header descriptions
ID_REF
VALUE raw Cq values. With single molecule Cq = 35

Data table
ID_REF VALUE
1 30.8
2 37.86
3 30.08
4 25.34
5 0
6 28.73
7 26.38
8 35.56
9 25.94
10 32.63
11 19.35
12 27.3
13 25.41
14 33.52
15 21.81
16 24.24
17 23.83
18 29.71
19 29.5
20 30.52

Total number of rows: 20801

Table truncated, full table size 228 Kbytes.




Supplementary data files not provided
Processed data not provided for this record
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