|
Status |
Public on Oct 03, 2014 |
Title |
2013-07-04_GPA2_CD8+ |
Sample type |
RNA |
|
|
Source name |
blood
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: granulomatotis with polyangiitis (GPA) cell type: primary t-cells facs: CD8+
|
Treatment protocol |
PBMCs were isolated by density gradient and subsequently sorted by employing anti CD4 and anti-CD8 antibodies using FACSAria
|
Growth protocol |
N/A
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extracted using miRVana (Ambion) according to the manufacturers guidelines
|
Label |
biotin
|
Label protocol |
according to the manufacturers guidelines (Affymetrix)
|
|
|
Hybridization protocol |
according to the manufacturers guidelines (Affymetrix)
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
Description |
mRNA expression profiles from primary cells
|
Data processing |
Sample data was processed using AGCC (Affymetrix default settings) and normalized using RMA
|
|
|
Submission date |
Apr 03, 2014 |
Last update date |
Oct 03, 2014 |
Contact name |
Robert Haesler |
E-mail(s) |
r.haesler@mucosa.de
|
Phone |
+49 (0) 431 597 5754
|
Organization name |
University Clinic of Schleswig Holstein
|
Department |
IKMB / 1st Dept. of Medicine
|
Lab |
Functional Genomics Platform
|
Street address |
Schittenhelmstr. 12
|
City |
Kiel |
ZIP/Postal code |
24105 |
Country |
Germany |
|
|
Platform ID |
GPL16686 |
Series (1) |
GSE56481 |
CD4+CD+8 double positive T-cell mRNA expression profiles from patients with granulomatotis with polyangiitis (GPA) compared to healthy controls |
|