|
| Status |
Public on Oct 03, 2014 |
| Title |
2013-07-04_VF_CD4+ |
| Sample type |
RNA |
| |
|
| Source name |
blood
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: healthy control
cell type: primary t-cells
facs: CD4+
|
| Treatment protocol |
PBMCs were isolated by density gradient and subsequently sorted by employing anti CD4 and anti-CD8 antibodies using FACSAria
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using miRVana (Ambion) according to the manufacturers guidelines
|
| Label |
biotin
|
| Label protocol |
according to the manufacturers guidelines (Affymetrix)
|
| |
|
| Hybridization protocol |
according to the manufacturers guidelines (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
mRNA expression profiles from primary cells
|
| Data processing |
Sample data was processed using AGCC (Affymetrix default settings) and normalized using RMA
|
| |
|
| Submission date |
Apr 03, 2014 |
| Last update date |
Oct 03, 2014 |
| Contact name |
Robert Haesler |
| E-mail(s) |
r.haesler@mucosa.de
|
| Phone |
+49 (0) 431 597 5754
|
| Organization name |
University Clinic of Schleswig Holstein
|
| Department |
IKMB / 1st Dept. of Medicine
|
| Lab |
Functional Genomics Platform
|
| Street address |
Schittenhelmstr. 12
|
| City |
Kiel |
| ZIP/Postal code |
24105 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE56481 |
CD4+CD+8 double positive T-cell mRNA expression profiles from patients with granulomatotis with polyangiitis (GPA) compared to healthy controls |
|
