|
| Status |
Public on Apr 16, 2015 |
| Title |
Patient 4 (6M) |
| Sample type |
RNA |
| |
|
| Source name |
Patient 4 (6M) after 6 Months treatment with Placebo along with Cardiac Medications
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: coronary artery disease (CAD)
cell type: PBMCs
|
| Treatment protocol |
PBMCs were isolated from whole blood of the study subjects by density gradient centrifugation method of Boyum , using Histopaque-1077 (Cat no.10771; Sigma, USA).
|
| Growth protocol |
PBMCs were isolated from 2 randomly selected patients in the each sub-group i.e. Terminalia arjuna or Placebo at 3 and 6 Months respectively to understand specificity, efficacy and mode of action of T.arjuna.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from samples with an RNeasy Mini Kit (Catalog No. 74104, QIAGEN, US) using silica column Method, as per manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA samples were prepared according to the standard Affymetrix protocol from 250ng total RNA (GeneChip®HT 3' IVT Express Kit, Affymetrix, USA).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of cRNA was hybridized for 16 hrs at 45°C on GeneChip®HT 3' IVT.
|
| Scan protocol |
The arrays were washed and stained after 16 hours of hybridization in Fluidic station 450 using appropriate Fluidic Script followed by the scanning of the Array to generate CEL files. Wash and Stain the arrays after 16 hours of hybridization in Fluidic station 450 by using appropriate Fluidic Script followed by the scanning of the Array to generate cell files.
|
| Description |
Gene Expression data from Patient after 6 Months Treatment with Placebo along with other Cardiac Medications after randomization at baseline
PBMCs were isolated from whole blood of the study subjects by the method of Boyum by density gradient centrifugation
|
| Data processing |
Raw gene expression data was analyzed using the GeneSpringGx 12.0 software (Agilent® Technologies, Santa Clara, CA)
Genes that showed more than 2-fold difference between the treated and untreated subjects and with a p-value of <0.05 were considered as differentially expressed.
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| |
|
| Submission date |
Apr 17, 2014 |
| Last update date |
Apr 16, 2015 |
| Contact name |
madavan vasudevan |
| E-mail(s) |
madavan.vasudevan@gmail.com
|
| Phone |
+91-9845093355
|
| Organization name |
Theomics International Private Limited
|
| Street address |
Kasturi Nagar
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560043 |
| Country |
India |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE56886 |
To evaluate the effect of Terminalia arjuna as an adjuvant therapy in subjects with stable coronary artery disease on standard dosage regimens versus placebo |
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