|
| Status |
Public on Oct 01, 2015 |
| Title |
IFN-α treated U6A cells+STAT2, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
U6A cells+WT STAT2
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: IFN
cell line: U6A (STAT2 null 2fTGH)
genotype/variation: WT
|
| Treatment protocol |
Tumor cells were seeded on 100mm dishes and left to adhere overnight. The next day cells were left untreated or treated with IFN-α (1000U/mL) or TNF-α (10ng/mL) for 4 hours at 37oC.
|
| Growth protocol |
Tumor cells were maintained in complete DMEM medium containing 10% fetal calf serum and antibiotics at 37oC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) and further purified by RNeasy kit (Qiagen, Valencia, CA). The quality of total RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA.
|
| |
|
| Hybridization protocol |
RNA samples were labeled and hybridized to the Affymetrix mouse Gene 2.0 ST Array according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
U6A cells+WT STAT2
|
| Data processing |
Affymetrix data were RMA-normalized using Affymetrix Expression Console Software, version 1.3.1
|
| |
|
| Submission date |
Apr 23, 2014 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Ana Maria Gamero |
| E-mail(s) |
gameroa@temple.edu
|
| Phone |
215-707-1268
|
| Organization name |
Temple University
|
| Department |
Biochemistry
|
| Street address |
3440 N. Broad St.
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19140 |
| Country |
USA |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE57017 |
Comparative Gene expression analysis of 2fTGH cells expressing Wild type STAT2 vs S734A-STAT2 in response to IFN-α or TNF-α |
|
