|
| Status |
Public on Apr 30, 2014 |
| Title |
RNA from BM MSC donor 3 |
| Sample type |
RNA |
| |
|
| Source name |
BM-derived MSC
|
| Organism |
Homo sapiens |
| Characteristics |
donor id: donor 3
tisse source for mscs: bone marrow (BM)
cell type: Mesenchymal Stromal Cells (MSC)
sample isolation method: plastic adherence
passage: passage 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from 5x10E6 cells using the Qiagen RNeasy PlusMini Kit.
|
| Label |
biotin
|
| Label protocol |
Bioting-labeling using standard Afymetrix WT Terminal Labeling Kit
|
| |
|
| Hybridization protocol |
Fragmented and labeled targets were hybridized overnight at 45C, 60rpm in an Affymetrix GeneChip Hybridization Oven 645.
|
| Scan protocol |
Genechips weres scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Data processing |
Arrays were normalized in R using oligolibrary. The script (R) used to generate the matrix is provided (data_processing_script.txt).
|
| |
|
| Submission date |
Apr 29, 2014 |
| Last update date |
Apr 30, 2014 |
| Contact name |
Andreas Reinisch |
| E-mail(s) |
reinisch@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Institute for Stem Cell Biology and Regenerative Medicine
|
| Street address |
265 Campus Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE57151 |
Epigenetic and in vivo comparison of diverse mesenchymal stromal cell sources reveals an endochonrdal signature for human hematopoietic niche formation |
|
