|
| Status |
Public on Dec 31, 2015 |
| Title |
D324_MINUS_---_xn cells |
| Sample type |
RNA |
| |
|
| Source name |
Wilms tumor cells
|
| Organism |
Homo sapiens |
| Characteristics |
developmental stage: adult
tissue: kidney
|
| Treatment protocol |
RNA samples were used to prepare biotinylated target DNA, according to manufacturer’s recommendations. The target complementary DNA (cDNA) generated from each sample was processed as per manufacturer’s recommendation using an Affymetrix GeneChip Instrument System
|
| Growth protocol |
Total RNA was extracted from each of the 8 samples: 7 Wilms' Xns and a normal fetal kidney using TRIZOL .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from each sample using TRIZOL .
|
| Label |
biotin
|
| Label protocol |
RNA samples were used to prepare biotinylated target DNA, according to manufacturer’s recommendations. The target complementary DNA (cDNA) generated from each sample was processed as per manufacturer’s recommendation using an Affymetrix GeneChip Instrument System
|
| |
|
| Hybridization protocol |
www.affymetrix.com/user/login.jsp?toURL=/support/file_download.affx?onloadforward=/support/downloads/manuals/3_ivt_express_kit_manual.pdf
|
| Scan protocol |
www.affymetrix.com/user/login.jsp?toURL=/support/file_download.affx?onloadforward=/support/downloads/manuals/3_ivt_express_kit_manual.pdf
|
| Data processing |
LINEAR RMA SIGNALS (PARTEK)
|
| |
|
| Submission date |
May 05, 2014 |
| Last update date |
Oct 25, 2022 |
| Contact name |
jasmine Jacob |
| E-mail(s) |
j-jacob@sheba.health.gov.il
|
| Phone |
0523790500
|
| Organization name |
Sheba Medical Center at Tel HaShomer
|
| Street address |
haChevel 33
|
| City |
Shoham |
| State/province |
-Select- |
| ZIP/Postal code |
60850 |
| Country |
Israel |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE57269 |
The Wilms' tumor blastema propagating cell is a self-renewing committed epithelial stem cell |
|
