|
| Status |
Public on Jan 01, 2015 |
| Title |
Infected_48h pi_Rep2 |
| Sample type |
RNA |
| |
|
| Source name |
CHME3 cells grown on transwells - Infected With JEV
|
| Organism |
Homo sapiens |
| Characteristics |
infection: Japanese encephalitis virus
time: 48 h
cell line: CHME3
cell type: Polarized cells
|
| Treatment protocol |
Infected with an MOI of 5 pfu/cell of Japanese encephalitis virus (P20778 strain )
|
| Growth protocol |
CHME3 cells grown on 6-wells plate
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA prepared using Qiagen RNA extraction kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human PrimeView Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from JEV infected human microglial cells CHME14
|
| Data processing |
The data were analyzed with Expression Console using Affymetrix default analysis settings and RMA as normalization method used. The probes of intensities less than the 20.0 percentile in the raw data were excluded.
|
| |
|
| Submission date |
May 06, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
ARUP BANERJEE |
| E-mail(s) |
banerjeea@thsti.res.in
|
| Phone |
9650328080
|
| Organization name |
TRANSLATIONAL HEALTH SCIENCE & TECHNOLOGY INSTITUTE
|
| Department |
VIDRC
|
| Lab |
THSTI, VIDRC
|
| Street address |
THSTI, 3RD MILE STONE, FARIDABAD GURGAON EXPRESSWAY, NCR BIOTECH CLUSTER
|
| City |
FARIDABAD |
| State/province |
Haryana |
| ZIP/Postal code |
121001 |
| Country |
India |
| |
|
| Platform ID |
GPL15207 |
| Series (2) |
| GSE57330 |
mRNA expression profile in CHME3 cells infected with Japanese encephalitis virus |
| GSE57647 |
Time- and phase-dependent expression profile of miRNA and mRNA in human microglial cells (CHME3) infected with Japanese encephalitis virus (JEV) P20778 strain |
|
