|
| Status |
Public on Aug 01, 2014 |
| Title |
human facial SKPs sample 2 |
| Sample type |
RNA |
| |
|
| Source name |
facial skin less than two years old cultured as SKPs
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: dermis
cell type: facial skin less than two years old cultured as SKPs
|
| Treatment protocol |
Cells proliferated as spheres. After one or two passages, spheres were placed in Trizol reagent.
|
| Growth protocol |
Epidermis was dissected from dermis following enzymatic digestion. The dermis was cut into small pieces and further digested and filtered until single cells were isolated. These cells were cultured in proliferation-promoting conditions as previously described.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed with the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Gene 2.0 ST Arrays (Affymetrix) and scanned at the HSC Microarray Facility.
|
| Scan protocol |
The hybridized microarray image was scanned with the GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data from <2 year old facial skin cultured as SKPs
|
| Data processing |
Raw probe intensity values were background corrected, normalized with quantile normalization, transformed into the log2 scale, and summarized into probesets using the Robust Multichip Analysis (RMA) algorithm at the gene level in the Affymetrix Expression Console program.
|
| |
|
| Submission date |
May 09, 2014 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Freda Miller |
| E-mail(s) |
fredam@sickkids.ca
|
| Organization name |
Hospital for Sick Children
|
| Street address |
555 University Avenue
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE57518 |
Direct genesis of functional rodent and human Schwann cells from skin mesenchymal precursors (human) |
| GSE57519 |
Direct genesis of functional rodent and human Schwann cells from skin mesenchymal precursors |
|
