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Sample GSM1384123 Query DataSets for GSM1384123
Status Public on Aug 01, 2014
Title human facial SKPs sample 3
Sample type RNA
 
Source name facial skin less than two years old cultured as SKPs
Organism Homo sapiens
Characteristics tissue: dermis
cell type: facial skin less than two years old cultured as SKPs
Treatment protocol Cells proliferated as spheres. After one or two passages, spheres were placed in Trizol reagent.
Growth protocol Epidermis was dissected from dermis following enzymatic digestion. The dermis was cut into small pieces and further digested and filtered until single cells were isolated. These cells were cultured in proliferation-promoting conditions as previously described.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed with the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions.
Label biotin
Label protocol Single-stranded cDNA was generated from the cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip Human Gene 2.0 ST Arrays (Affymetrix) and scanned at the HSC Microarray Facility.
Scan protocol The hybridized microarray image was scanned with the GeneChip Scanner 3000 7G (Affymetrix).
Description Gene expression data from <2 year old facial skin cultured as SKPs
Data processing Raw probe intensity values were background corrected, normalized with quantile normalization, transformed into the log2 scale, and summarized into probesets using the Robust Multichip Analysis (RMA) algorithm at the gene level in the Affymetrix Expression Console program.
 
Submission date May 09, 2014
Last update date Aug 01, 2014
Contact name Freda Miller
E-mail(s) fredam@sickkids.ca
Organization name Hospital for Sick Children
Street address 555 University Avenue
City Toronto
State/province ON
ZIP/Postal code M5G1L7
Country Canada
 
Platform ID GPL16686
Series (2)
GSE57518 Direct genesis of functional rodent and human Schwann cells from skin mesenchymal precursors (human)
GSE57519 Direct genesis of functional rodent and human Schwann cells from skin mesenchymal precursors

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
16650001 1.98258
16650003 2.52825
16650005 4.93537
16650007 2.20312
16650009 1.26893
16650011 2.87156
16650013 5.74042
16650015 5.3525
16650017 5.45266
16650019 5.60479
16650021 5.84356
16650023 3.14442
16650025 4.83614
16650027 2.84232
16650029 4.69199
16650031 6.51588
16650033 1.13576
16650035 1.74784
16650037 1.24615
16650041 7.24879

Total number of rows: 53617

Table truncated, full table size 886 Kbytes.




Supplementary file Size Download File type/resource
GSM1384123_HuGene2_012214H_FM3_C3.CEL.gz 8.2 Mb (ftp)(http) CEL
GSM1384123_HuGene2_012214H_FM3_C3.rma-gene-full.chp.gz 353.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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