|
| Status |
Public on Jan 18, 2015 |
| Title |
Genome-engineered organoid4 |
| Sample type |
RNA |
| |
|
| Source name |
genome-engineered organoids from human colon organoids
|
| Organism |
Homo sapiens |
| Characteristics |
genetic modification: AKST (CRISPR/Cas9)
tissue: genome-engineered organoids from human colon organoids
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Dissociated single cells derived from indicated organoids were cultured for 7 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA with Rneasy Plus Mini Kit (QIAGEN) was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (3'IVT Express Kit User Manual,Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip PrimeView Human Gene Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
genome engineered organoids
|
| Data processing |
The data were analyzed with GeneSpring GX 12 using RMA as summarization algorithm and baseline to median of all samples.
|
| |
|
| Submission date |
May 26, 2014 |
| Last update date |
Feb 26, 2018 |
| Contact name |
Mariko Shimokawa |
| Organization name |
Keio University
|
| Street address |
35, Shinanomachi, Shinjuku-Ku
|
| City |
Tokyo |
| ZIP/Postal code |
160-8582 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE57965 |
mRNA expression data from adenoma, gene modified, and colorectal cancer organoid. |
|
