|
| Status |
Public on Jun 22, 2015 |
| Title |
H1650 PLKO CONTROL replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
NCI-H1650 pLKO control
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1650
cell type: Lung cancer cell line
genotype/variation: empty vector pLKO control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Human PrimeView Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
HuPV_072513H_WL9_I.rma-Signal
|
| Data processing |
The data were analyzed with Expression Console using Affymetrix default analysis settings and RMA normalization method.
|
| |
|
| Submission date |
May 26, 2014 |
| Last update date |
Jun 22, 2015 |
| Contact name |
Roland Hubaux |
| E-mail(s) |
rhubaux@straticell.com
|
| Phone |
003281728586
|
| Organization name |
StratiCELL
|
| Department |
R&D
|
| Street address |
rue Jean Sonet 10
|
| City |
Isnes Gembloux |
| State/province |
Namur |
| ZIP/Postal code |
5032 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE57966 |
Microtubule Affinity-Regulating Kinase 2 is associated with DNA damage response and cisplatin resistance in non-small cell lung cancer. |
|
