|
| Status |
Public on May 08, 2015 |
| Title |
WiDr_shIKKa_2 |
| Sample type |
RNA |
| |
|
| Source name |
WiDr human colorectal cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: WiDr
condition: shIKKa
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted following the RNA-easy Quiagen protocol.
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA from each sample was processed, labelled according to the Affymetrix GeneChip® Whole Transcript Sense Target Labelling Assay
|
| |
|
| Hybridization protocol |
Sample was hybridized to Affymetrix PrimeView array using a Affymetrix GeneChip Hybridization Oven 645
|
| Scan protocol |
GeneChip was scanned using Affymetrix GeneChip Scanner 3000 7G
|
| Description |
CRC cell lines
Gene expression data from control sh-IKKalpha-transduced WiDr cells
|
| Data processing |
Data were analyzed using the RMA algorithm and then LIMMA was applied to calculate significant differential expression between samples. This was performed in R (v 3.0.2) with aroma.affymetrix and bioconductor packages
|
| |
|
| Submission date |
May 27, 2014 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Júlia Perera-Bel |
| E-mail(s) |
mardata-bu@researchmar.net
|
| Organization name |
Hospital del Mar Research Institute
|
| Department |
MARData-BU
|
| Street address |
Doctor Aiguader, 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE57979 |
BRAF-induced tumorigenesis is IKKalpha dependent but NFκB independent |
|
