|
| Status |
Public on Jan 01, 2015 |
| Title |
Pu.1_ChIPseq rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Dendritic cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Monocyte-derived denditric cells
chip antibody: PU.1 SC-352 from SantaCruz
|
| Growth protocol |
monocytes were cultured in RPMI 10% FCS supplemented with 2-mercaptoethanol (50 μM), GM-CSF (600 U/ml), and IL-4 (750 U/ml)
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Chromatine was isolated, fragmented by sonication and immunoprecipitate it with anti PU1 antibody
Libraries were prepared according to Illumina's instructions using the Truseq ChIP-seq protocol.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Basecalls performed using CASAVA version 1.8.2
Reads were mapped to the reference genome using bowtie 0.12.7 with -m 1
Peaks were called using macs2 2.0.10.20130520 with -p 0.1
Genome_build: GRCh37.71
|
| |
|
| Submission date |
Jun 26, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Sylvain LEMEILLE |
| Organization name |
University of Geneva Medical School
|
| Department |
Department of Pathology and Immunology
|
| Street address |
1 rue Michel-Servet
|
| City |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE58864 |
Extensive remodeling of DC function by rapid maturation-induced epigenetic gene silencing |
|
| Relations |
| BioSample |
SAMN02887301 |
| SRA |
SRX627430 |
| Named Annotation |
GSM1421019_3320h_vs_GRCh37.71_peaks.bed.gz |
| Named Annotation |
GSM1421019_3320hPU_vs_GRCh37.71.bw |
