|
| Status |
Public on Aug 01, 2014 |
| Title |
NOK cl1 pTURF transfected p23 |
| Sample type |
RNA |
| |
|
| Source name |
NOK vector transfected
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hTert immortalized normal oral keratinocytes derived from gingiva
infection: vector transfected
|
| Treatment protocol |
Uninfected parental cells, vector control (pTVRF=PTURF), EBV-infected, and EBV-negative transiently-infected clones were seeded at 1x10^6 cells in a T75 flask. RNA was harvested 3 days after seeding using STAT60 (Teltest) following the recommended protocol.
|
| Growth protocol |
Human telomerase (hTERT)-immortalized normal oral keratinocytes (NOK, kindly gifted by Dr. Karl Munger) were grown in keratinocyte serum free media (KSFM) supplemented with human epidermal growth factor and bovine pituitary extract (Life Technologies) in a humidified CO2 incubator at 37oC. Cells were passaged once a week using trypsinization at a 1:4 split.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Stat-60 (TEL-TEST) as per manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared from 150 ng of total RNA using the Affymetrix GeneChip 3' IVT Express Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA was hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G with autoloader.
|
| Description |
NOK pTVRF cl2 p23
|
| Data processing |
The data were analyzed with Expression Console version 1.3 (MAS 5 algorithm) using Affymetrix default analysis settings and global scaling with a target intensity value of 500 as normalization method.
|
| |
|
| Submission date |
Jun 30, 2014 |
| Last update date |
Aug 01, 2014 |
| Contact name |
Rona S. Scott |
| E-mail(s) |
rscott1@lsuhsc.edu
|
| Phone |
318-675-6263
|
| Organization name |
Louisiana State University Health Sciences Center-Shreveport
|
| Department |
Microbiology and Immunology
|
| Street address |
1501 Kings Highway
|
| City |
Shreveport |
| State/province |
LA |
| ZIP/Postal code |
71130 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE58914 |
Expression data from Epstein-Barr virus infection of immortalized normal oral keratinocytes |
| GSE59843 |
Genome wide DNA methylation and expression profiling of Epstein-Barr virus infected immortalized normal oral keratinocytes |
|
