|
| Status |
Public on Jul 09, 2014 |
| Title |
Wild-type animal 1 |
| Sample type |
SRA |
| |
|
| Source name |
E12.5 dorsal cortex
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: E12.5
tissue: brain/dorsal cortex
background strain: C57BL/6J
|
| Growth protocol |
mice were maintained using standard husbandry protocols.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
embryonic cortices were removed, and total RNA was harvested using Rneasy mini kit (Qiagen).
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm9 whole genome and RPKM calculated using Gobyweb1.7
Genome_build: mm9
Supplementary_files_format_and_content: wig and bed files
|
| |
|
| Submission date |
Jul 08, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrew Pollock |
| Organization name |
Weill Cornell Medical College
|
| Department |
Cell and Developmental Biology
|
| Lab |
Sun Lab
|
| Street address |
1300 York Ave
|
| City |
new york |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL15103 |
| Series (1) |
| GSE59204 |
miR-7 blockade alters E12.5 mouse cortical mRNA levels |
|
| Relations |
| BioSample |
SAMN02905002 |
| SRA |
SRX648109 |
