|
| Status |
Public on Jan 01, 2015 |
| Title |
CRTC1 n2 |
| Sample type |
RNA |
| |
|
| Source name |
Caco-2, CRTC1 overexpression
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Caco-2
cell type: epithelial colonic tumor cell line
expression vector: CRTC1
|
| Treatment protocol |
Subconfluent Caco-2 cells were transfected with 200ng of CRTC1 expression plasmid or corresponding empty vector (pBS) overnight.
|
| Growth protocol |
Caco-2 clone TC7 cells (kind gift of Monique Rousset, Paris, France) were grown in DMEM High Glucose (4.5g/l) medium supplemented with 10% serum and 1% penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of mRNA from cultured cells were performed by using RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from total RNA (following the GeneChip Whole Transcript (WT) Sense Target labelling assay kit Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA were hybridized for 16 hr at 45C on Affymetrix Human Gene 2.0 ST Arrays. Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix).
|
| Description |
Sample name: 4_CRTC1
Gene expression data from Caco-2 epithelial colonic tumor cell with CRTC1 overexpression.
|
| Data processing |
The data were preprocessed and analyzed using Partek Genomic Suite.
|
| |
|
| Submission date |
Jul 24, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Florent Dumont |
| E-mail(s) |
florent.dumont@u-psud.fr
|
| Organization name |
Université Paris-Saclay
|
| Street address |
17 avenue des sciences
|
| City |
91400 Orsay |
| State/province |
Ile de France |
| ZIP/Postal code |
91400 |
| Country |
France |
| |
|
| Platform ID |
GPL17930 |
| Series (1) |
| GSE59730 |
CRTC1 overexpression in Caco-2 colon cancer cells |
|
