|
| Status |
Public on Oct 30, 2015 |
| Title |
Normal control, rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
Cerebellum, normal control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: cerebellum
diagnosis: free of known CNS neurological disease
age: age-matched (average age 25 yrs)
|
| Treatment protocol |
Frozen samples were not treated.
|
| Growth protocol |
Human frozen tissue was obtained from the NICHD Brain and Tissue Bank of Developmental Disorders at the University of Maryland, Baltimore, MD.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was extracted with an RNeasy kit from Qiagen, Inc.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Total cellular RNA
Replicate 4
|
| Data processing |
Output from Illumina GenomeStudio was loaded into R using limma, normalized with the neqc function (background correction followed by quantile normalization), and then modeled using lmFit and eBayes functions in limma.
|
| |
|
| Submission date |
Sep 02, 2014 |
| Last update date |
Oct 30, 2015 |
| Contact name |
Ronald P. Hart |
| E-mail(s) |
rhart@rutgers.edu
|
| Phone |
848-445-1783
|
| Organization name |
Rutgers University
|
| Department |
Cell Biology & Neuroscience
|
| Street address |
604 Allison Rd Rm B430
|
| City |
Piscataway |
| State/province |
NJ |
| ZIP/Postal code |
08854 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE61019 |
Ataxia-telangiectasia cerebellar cortex expression |
| GSE61169 |
Selective loss of 5hmC links to A-T Purkinje cell vulnerability |
|
