|
Status |
Public on Nov 06, 2014 |
Title |
BJ-iPS-M |
Sample type |
genomic |
|
|
Source name |
RNA-reprogrammed induced pluripotent stem cell line derived from neonatal BJ fibroblasts, clone M
|
Organism |
Homo sapiens |
Characteristics |
cell line: RNA-reprogrammed induced pluripotent stem cell line derived from neonatal BJ fibroblasts, clone M cell type: Undifferentiated induced pluripotent stem cells
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated (High Pure PCR Template Preparation Kit, Roche) and bisulfite converted (EZDNA Methylation Kit, Zymo Research) according to the manufacturers' protocol
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina protocol
|
|
|
Hybridization protocol |
Standard Illumina protocol
|
Scan protocol |
HumanMethylation450 BeadChips were scanned with Illumina's iScan System
|
Description |
9376538060_R05C01
|
Data processing |
IDAT files were processed and normalized by subset-quantile within array normalization (SWAN) and adjusted for batch effects using the R package ChAMP (version 1.2.7)
|
|
|
Submission date |
Sep 16, 2014 |
Last update date |
Nov 06, 2014 |
Contact name |
Ido Sagi |
Organization name |
The Hebrew University of Jerusalem
|
Department |
Genetics
|
Lab |
Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
|
Street address |
Givat Ram
|
City |
Jerusalem |
ZIP/Postal code |
91904 |
Country |
Israel |
|
|
Platform ID |
GPL13534 |
Series (2) |
GSE61461 |
Coding mutations and loss-of-imprinting in human pluripotent cells derived by nuclear transfer and defined factors [DNA methylation profiling] |
GSE61657 |
Coding mutations and loss-of-imprinting in human pluripotent cells derived by nuclear transfer and defined factors |
|