|
Status |
Public on Nov 06, 2014 |
Title |
BJ fibroblasts |
Sample type |
SRA |
|
|
Source name |
BJ fibroblast cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: BJ-Fib cell type: Fibroblasts
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA was isolated using the Total RNA Purification Micro Kit (Norgen). For RNA sequencing analysis, poly(A) pull-down was used to enrich mRNAs from total RNA samples (200 ng–1 µg per sample, RIN > 8) Libraries were prepared using the Illumina TruSeq RNA prep kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Reads were mapped to the human reference genome (NCBI/build37.2) using Tophat (version 2.0.4) Fragments per kilobase of transcript per million mapped reads (FPKM) values were calculated using Cufflinks (version 2.0.2) Genome_build: NCBI/build 37.2 Supplementary_files_format_and_content: Tab-delimited text file includes FPKM values for each sample
|
|
|
Submission date |
Sep 23, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Ido Sagi |
Organization name |
The Hebrew University of Jerusalem
|
Department |
Genetics
|
Lab |
Nissim Benvenisty Lab, The Azrieli Center for Stem Cells and Genetic Research
|
Street address |
Givat Ram
|
City |
Jerusalem |
ZIP/Postal code |
91904 |
Country |
Israel |
|
|
Platform ID |
GPL16791 |
Series (2) |
GSE61656 |
Coding mutations and loss-of-imprinting in human pluripotent cells derived by nuclear transfer and defined factors [RNA-Seq] |
GSE61657 |
Coding mutations and loss-of-imprinting in human pluripotent cells derived by nuclear transfer and defined factors |
|
Relations |
BioSample |
SAMN03075773 |
SRA |
SRX708530 |