|
Status |
Public on May 14, 2015 |
Title |
DMSO-polyIC |
Sample type |
RNA |
|
|
Source name |
RAW264.7 cells treated with DMSO and polyI:C
|
Organism |
Mus musculus |
Characteristics |
cell line: RAW264.7 treatment: DMSO and polyI:C
|
Treatment protocol |
RAW 264.7 cells were pre-treated with DMSO or 100nM of 9cis-retinoic acid for 16hours. The cells were then stimulated with polyI:C (6ug transfected) or LPS (500ng/mL) for 2 hours.
|
Growth protocol |
RAW264.7 were grown in DMEM containing 10%FBS and 1% penicillin/streptomycin, 37C and 10%CO2
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by TRIzol
|
Label |
biotin
|
Label protocol |
Biotin labeled to Affymetrix Mouse Genome 430.2 GeneChip microarrays (Affymetrix Inc., Santa Clara, CA, USA) using Affymetrix reagents and protocols (http://www.affymetrix.com).
|
|
|
Hybridization protocol |
Affymetrix Mouse Genome 430.2 GeneChip microarrays (Affymetrix Inc., Santa Clara, CA, USA)
|
Scan protocol |
Affymetrix standard protocols
|
Data processing |
Microarray data were normalized using GCRMA
|
|
|
Submission date |
Oct 06, 2014 |
Last update date |
May 14, 2015 |
Contact name |
feng ma |
Organization name |
CAMS
|
Department |
Suzhou Institute of Systems Medicine
|
Lab |
MaF Lab
|
Street address |
218 Xinghu Street, B5-527
|
City |
Suzhou |
State/province |
Jiangsu |
ZIP/Postal code |
215123 |
Country |
China |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE62107 |
Gene expression during ligands activation of RXR in activated RAW264.7 cells |
|