|
| Status |
Public on May 14, 2015 |
| Title |
DMSO-polyIC |
| Sample type |
RNA |
| |
|
| Source name |
RAW264.7 cells treated with DMSO and polyI:C
|
| Organism |
Mus musculus |
| Characteristics |
cell line: RAW264.7
treatment: DMSO and polyI:C
|
| Treatment protocol |
RAW 264.7 cells were pre-treated with DMSO or 100nM of 9cis-retinoic acid for 16hours. The cells were then stimulated with polyI:C (6ug transfected) or LPS (500ng/mL) for 2 hours.
|
| Growth protocol |
RAW264.7 were grown in DMEM containing 10%FBS and 1% penicillin/streptomycin, 37C and 10%CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by TRIzol
|
| Label |
biotin
|
| Label protocol |
Biotin labeled to Affymetrix Mouse Genome 430.2 GeneChip microarrays (Affymetrix Inc., Santa Clara, CA, USA) using Affymetrix reagents and protocols (http://www.affymetrix.com).
|
| |
|
| Hybridization protocol |
Affymetrix Mouse Genome 430.2 GeneChip microarrays (Affymetrix Inc., Santa Clara, CA, USA)
|
| Scan protocol |
Affymetrix standard protocols
|
| Data processing |
Microarray data were normalized using GCRMA
|
| |
|
| Submission date |
Oct 06, 2014 |
| Last update date |
May 14, 2015 |
| Contact name |
feng ma |
| Organization name |
CAMS
|
| Department |
Suzhou Institute of Systems Medicine
|
| Lab |
MaF Lab
|
| Street address |
218 Xinghu Street, B5-527
|
| City |
Suzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
215123 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE62107 |
Gene expression during ligands activation of RXR in activated RAW264.7 cells |
|
