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Sample GSM1519712 Query DataSets for GSM1519712
Status Public on May 14, 2015
Title DMSO-polyIC
Sample type RNA
 
Source name RAW264.7 cells treated with DMSO and polyI:C
Organism Mus musculus
Characteristics cell line: RAW264.7
treatment: DMSO and polyI:C
Treatment protocol RAW 264.7 cells were pre-treated with DMSO or 100nM of 9cis-retinoic acid for 16hours. The cells were then stimulated with polyI:C (6ug transfected) or LPS (500ng/mL) for 2 hours.
Growth protocol RAW264.7 were grown in DMEM containing 10%FBS and 1% penicillin/streptomycin, 37C and 10%CO2
Extracted molecule total RNA
Extraction protocol Total RNA was extracted by TRIzol
Label biotin
Label protocol Biotin labeled to Affymetrix Mouse Genome 430.2 GeneChip microarrays (Affymetrix Inc., Santa Clara, CA, USA) using Affymetrix reagents and protocols (http://www.affymetrix.com).
 
Hybridization protocol Affymetrix Mouse Genome 430.2 GeneChip microarrays (Affymetrix Inc., Santa Clara, CA, USA)
Scan protocol Affymetrix standard protocols
Data processing Microarray data were normalized using GCRMA
 
Submission date Oct 06, 2014
Last update date May 14, 2015
Contact name feng ma
Organization name CAMS
Department Suzhou Institute of Systems Medicine
Lab MaF Lab
Street address 218 Xinghu Street, B5-527
City Suzhou
State/province Jiangsu
ZIP/Postal code 215123
Country China
 
Platform ID GPL1261
Series (1)
GSE62107 Gene expression during ligands activation of RXR in activated RAW264.7 cells

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 299.353 P 8.14279e-05
AFFX-BioB-M_at 429.228 P 4.42873e-05
AFFX-BioB-3_at 283.57 P 7.00668e-05
AFFX-BioC-5_at 1299.09 P 5.16732e-05
AFFX-BioC-3_at 1420.26 P 4.42873e-05
AFFX-BioDn-5_at 3105.9 P 4.42873e-05
AFFX-BioDn-3_at 6121.96 P 8.14279e-05
AFFX-CreX-5_at 20971.8 P 5.16732e-05
AFFX-CreX-3_at 22497.8 P 4.42873e-05
AFFX-DapX-5_at 10.4656 A 0.52976
AFFX-DapX-M_at 3.06363 A 0.659339
AFFX-DapX-3_at 19.7546 A 0.686277
AFFX-LysX-5_at 1.22809 A 0.966323
AFFX-LysX-M_at 10.0269 A 0.659352
AFFX-LysX-3_at 2.1049 A 0.876428
AFFX-PheX-5_at 1.22062 A 0.981719
AFFX-PheX-M_at 2.57952 A 0.897835
AFFX-PheX-3_at 42.9536 A 0.083592
AFFX-ThrX-5_at 14.6704 A 0.250796
AFFX-ThrX-M_at 16.7572 A 0.411373

Total number of rows: 45101

Table truncated, full table size 1376 Kbytes.




Supplementary file Size Download File type/resource
GSM1519712_D-P.CEL.gz 3.6 Mb (ftp)(http) CEL
GSM1519712_D-P.mas5.CHP.gz 396.6 Kb (ftp)(http) CHP
Processed data included within Sample table

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