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Sample GSM1522124 Query DataSets for GSM1522124
Status Public on May 25, 2015
Title Microglia_specificpathogenfree_6hr_after_LPS_ic_3
Sample type RNA
 
Source name Microglia, LPS i.c., SPF
Organism Mus musculus
Characteristics cell type: microglia
treatment: LPS i.c.
condition: specifc pathogen free (SPF)
age: 6-8 weeks old
strain: C57BL/6
Treatment protocol 5 µg LPS were diluted in 10 µl PBS and applied intracranially under isofluoran anesthesia. Animals returned to their cages once they had recovered their righting reflex. 6 hours later, animals were analyzed. Control animals were injected with 10 µl sterile PBS i.c.
Growth protocol Mice were housed under SPF or germ-free conditions. Animals were 6-8 weeks old.
Extracted molecule total RNA
Extraction protocol Adult microglia were harvested using density gradient separation. CD11b+CD45lo microglial cell populations were FACS-sorted and collected directly in cell lysis buffer and subsequently RNA was isolated with the Arcturus Pico Pure RNA Isolation Kit (Life Technologies) according to the manufacturer's protocol.
Label Biotin
Label protocol Total RNA was amplified using the Ovation PicoSL WTA System V2 and further processed using the Encore Biotin Module according to the instructions supplied by the manufacturer (NuGen).
 
Hybridization protocol Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
Scan protocol Biotinylated cRNA was hybridized on GeneChip® Mouse Gene 2.1 ST Arrays , that were stained, washed and scanned following standard procedures.
Description Microglia from specifc pathogen free (SPF) mice treated with LPS i.c.
Data processing Background correction and normalization was performed using the RMA-Sketch implementation in Affymetrix Expression Console
 
Submission date Oct 09, 2014
Last update date May 25, 2015
Contact name Ori Staszewski
E-mail(s) ori.staszewski@uniklinik-freiburg.de
Organization name University Medical Center Freiburg
Department Institute of Neuropathology
Street address Breisacher Str. 64
City Freiburg
ZIP/Postal code D-79106
Country Germany
 
Platform ID GPL17400
Series (2)
GSE62201 Host microbiota constantly control maturation and function of microglia in the central nervous system (part 1)
GSE67858 Host microbiota constantly control maturation and function of microglia in the central nervous system

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
17200001 2.38898
17200003 5.06883
17200005 3.18678
17200007 3.65373
17200009 3.23833
17200011 4.22742
17200013 5.07225
17200015 3.9243
17200017 2.76527
17200019 1.63273
17200021 2.53987
17200023 4.66315
17200025 3.17302
17200027 4.32058
17200029 2.35171
17200031 3.65106
17200033 3.86473
17200035 3.42475
17200037 1.51335
17200039 2.01826

Total number of rows: 41345

Table truncated, full table size 682 Kbytes.




Supplementary file Size Download File type/resource
GSM1522124_A912h_06_Mg_SPF_LPS_3.CEL.gz 4.8 Mb (ftp)(http) CEL
GSM1522124_A912h_06_Mg_SPF_LPS_3.rma-gene-full.chp.gz 256.9 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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