|
| Status |
Public on Sep 01, 2018 |
| Title |
LNCaP, replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: androgen-sensitive human prostate adenocarcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol.
|
| Label |
SYBR Green
|
| Label protocol |
cDNA was synthesised from 1ug total RNA using Superscript 3 Reverse Transcriptase (Life Technologies) and Quantitative real-time PCR performed using an Applied Biosciences 7900HT following the Manufacturer’s instructions. Primers were obtained from Integrated DNA Technologies.
|
| |
|
| Hybridization protocol |
N/A
|
| Scan protocol |
N/A
|
| Description |
Cancer
LNCaP.2
|
| Data processing |
Mean target gene signals were normalised to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)]
The 'averaged_normalized.txt' reports normalised mean signal (against housekeeping gene ALAS1).
All deltadeltaCt based fold-change calculations were performed in the R statistical package.
the 'fold_change.txt' reports test/control (i.e., LNCaP/PrEC) ratios.
Please note that sample data table contains raw Ct values
|
| |
|
| Submission date |
Oct 16, 2014 |
| Last update date |
Sep 01, 2018 |
| Contact name |
Timothy John Peters |
| E-mail(s) |
t.peters@garvan.org.au
|
| Phone |
+61292958325
|
| Organization name |
Garvan Institute of Medical Research
|
| Department |
Immunology
|
| Lab |
Immunogenomics
|
| Street address |
384 Victoria St
|
| City |
Darlinghurst |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
| |
|
| Platform ID |
GPL19299 |
| Series (2) |
| GSE62409 |
Real-time quantitative PCR analysis of Normal and Cancerous Prostate Cells [RT-PCR] |
| GSE62410 |
Transcriptome profiling of Normal and Cancerous Prostate Cells |
|
