|
| Status |
Public on Sep 01, 2018 |
| Title |
LNCaP, untreated [gene-level] |
| Sample type |
RNA |
| |
|
| Source name |
androgen-sensitive human prostate adenocarcinoma (LNCaP)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
|
| Treatment protocol |
Untreated.
|
| Growth protocol |
LNCaP cells were grown at 37ºC with 5% CO2 in T medium with 10% fetal calf serum. PrEC cells were grown at 37ºC with 5% CO2 in PREGM media (Cambrex).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol at the Ramachiotti Center for Gene Function Analysis, UNSW, Australia.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the standard Affymetrix protocol at the Ramachiotti Center for Gene Function Analysis, UNSW, Australia.
|
| Scan protocol |
Human Transciptome arrays were scanned using a GC3000 scanner at the Ramaciotti Centre for Gene Function Analysis at the University of New South Wales, Kensington, NSW, Australia), according to the manufacturers instructions.
|
| Description |
HF_LNCaP_(HTA-2_0).CEL
|
| Data processing |
The data were RMA normalized Using Expression Console (from Affymetrix and the library files on the Affymetrix website). The analyses details/parameters are included in the readme.txt.
|
| |
|
| Submission date |
Oct 20, 2014 |
| Last update date |
Sep 01, 2018 |
| Contact name |
Timothy John Peters |
| E-mail(s) |
t.peters@garvan.org.au
|
| Phone |
+61292958325
|
| Organization name |
Garvan Institute of Medical Research
|
| Department |
Immunology
|
| Lab |
Immunogenomics
|
| Street address |
384 Victoria St
|
| City |
Darlinghurst |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
| |
|
| Platform ID |
GPL17586 |
| Series (2) |
| GSE62410 |
Transcriptome profiling of Normal and Cancerous Prostate Cells |
| GSE62497 |
Transcriptome profiling of Normal and Cancerous Prostate Cells using Affymetrix Human Transcriptome Array 2.0 |
|
