|
| Status |
Public on Dec 30, 2015 |
| Title |
IgG ChIP |
| Sample type |
SRA |
| |
|
| Source name |
ckit and sca1 positive cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Hematopoietic stem and progenitor cells from mouse bone marrow
chip antibody: IgG
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ckit and sca1 positive cells were isolated from the bone marrow of Mx1 Cre:Usp16loxp/loxp mice. DNA was sheared into approximately 200bp fragments by sonication. Usp16 bound DNA was immunoprecipitated by standard protocols.
Libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 1000 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Reads were mapped to mm10 with Bowtie. Duplicate reads were removed with Picard. Peaks were called with MACS.
Genome_build: mm10
Supplementary_files_format_and_content: peak
|
| |
|
| Submission date |
Oct 29, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Hengbin Wang |
| Organization name |
Virginia Commonwealth University
|
| Department |
Massey Cancer Institute
|
| Street address |
401 College Street
|
| City |
Richmond |
| State/province |
VA |
| ZIP/Postal code |
23298 |
| Country |
USA |
| |
|
| Platform ID |
GPL15103 |
| Series (2) |
|
| Relations |
| BioSample |
SAMN03151601 |
| SRA |
SRX746910 |
