|
| Status |
Public on Mar 01, 2015 |
| Title |
Ago2 IP miR-191 BioRep2_chip |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cell_transfected with miR-191 duplex
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
antibody: Ago2 (Abcam ab57113)
|
| Treatment protocol |
mature miR-191 duplex at a concentration of 100nM was transfected with lipofectamine 2000
|
| Growth protocol |
DMEM supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TRIzol reagent
|
| Label |
Cy3
|
| Label protocol |
Standard Illumina TotalPrep RNA Amplification Kit protocol
|
| |
|
| Hybridization protocol |
Standard Illumina TotalPrep RNA Amplification Kit protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
RNA isolated from Ago2 immunoprecipitation
|
| Data processing |
Quantile normalized using Illumina BeadStudio
|
| |
|
| Submission date |
Nov 19, 2014 |
| Last update date |
Mar 01, 2015 |
| Contact name |
Vishy Iyer |
| E-mail(s) |
iyerlab@gmail.com
|
| Phone |
5122327833
|
| Organization name |
University of Texas at Austin
|
| Department |
Molecular Biosciences
|
| Street address |
2500 Speedway Dr. MBB 3.212
|
| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78712 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE63483 |
Genome-wide hsa-miR-191 target profile [chip] |
| GSE63556 |
Genome wide miR-191 target profile determined by RIP and gene expression profiling |
|
