|
Status |
Public on Sep 08, 2015 |
Title |
Day 21 NPC + EC + MSC Plate 1 s2 |
Sample type |
SRA |
|
|
Source name |
H1 human embryonic stem cell derived neural progenitor cells, endothelial cells, and mesenchymal stem cells
|
Organism |
Homo sapiens |
Characteristics |
screening chemicals: n/a
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen Rneasy mini kit with RLT lysis buffer TruSeq and custom library prep
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
Human neural progenitor cells were cultured on PEG hydrogel in N2B27F2 media, endothelial cells and mesenchymal cells were then seeded on top of the NPC on day 9. Cells were lysed for RNA purification on day21. 131212_multiplexing_Lanes23_E1_NPC_100K_plus_MSCinMSC_plus_EC_D21_minus_2
|
Data processing |
Basecalls performed using CASAVA version 1.8.2 Gene expression estimates obtained using RSEM v 1.2.3, with Bowtie 0.12.9 for alignment. Genome Reference: hg19 Supplementary_files_format_and_content: RSEM TPMs (Gene expression levels)
|
|
|
Submission date |
Dec 06, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Peng Jiang |
E-mail(s) |
p.jiang@csuohio.edu
|
Organization name |
Cleveland State University
|
Street address |
2121 Euclid Ave
|
City |
Cleveland |
State/province |
OH |
ZIP/Postal code |
44115 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE63935 |
Human pluripotent stem cell-derived neural constructs for predictive neurotoxicity screening |
|
Relations |
BioSample |
SAMN03254691 |
SRA |
SRX798688 |