|
| Status |
Public on Sep 08, 2015 |
| Title |
H1 Human Embryonic Stem Cells Sample 4 |
| Sample type |
SRA |
| |
|
| Source name |
H1 human embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
screening chemicals: n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy mini kit with RLT lysis buffer
TruSeq and custom library prep
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Human embrynoic stem cell H1 were cultured in E8 media and harvested for RNA.
131227_multiplexing_Lanes3_Sample_38463_000192
|
| Data processing |
Basecalls performed using CASAVA version 1.8.2
Gene expression estimates obtained using RSEM v 1.2.3, with Bowtie 0.12.9 for alignment.
Genome Reference: hg19
Supplementary_files_format_and_content: RSEM TPMs (Gene expression levels)
|
| |
|
| Submission date |
Dec 06, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Peng Jiang |
| E-mail(s) |
p.jiang@csuohio.edu
|
| Organization name |
Cleveland State University
|
| Street address |
2121 Euclid Ave
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44115 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE63935 |
Human pluripotent stem cell-derived neural constructs for predictive neurotoxicity screening |
|
| Relations |
| BioSample |
SAMN03254759 |
| SRA |
SRX798694 |
