|
| Status |
Public on Sep 08, 2015 |
| Title |
Day 21 c24b |
| Sample type |
SRA |
| |
|
| Source name |
H1 human embryonic stem cell derived neural progenitor cells, endothelial cells, mesenchymal stem cells, and microglia/macrophage precursors
|
| Organism |
Homo sapiens |
| Characteristics |
screening chemicals: 4-hydroxy-tamoxifen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy mini kit with RLT lysis buffer
TruSeq and custom library prep
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Human neural progenitor cells were cultured on PEG hydrogel in N2B27F2 media, endothelial cells and mesenchymal cells were then seeded on top of the NPC on day 9. Microglia/macrophage progenitors were seeded on day 13. Chemicals exposure started on day 14. Cells were lysed for RNA purification on day 21.
140304_multiplexing_Lanes123456_d7c24b
|
| Data processing |
Basecalls performed using CASAVA version 1.8.2
Gene expression estimates obtained using RSEM v 1.2.3, with Bowtie 0.12.9 for alignment.
Genome Reference: hg19
Supplementary_files_format_and_content: RSEM TPMs (Gene expression levels)
|
| |
|
| Submission date |
Dec 06, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Peng Jiang |
| E-mail(s) |
p.jiang@csuohio.edu
|
| Organization name |
Cleveland State University
|
| Street address |
2121 Euclid Ave
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44115 |
| Country |
USA |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE63935 |
Human pluripotent stem cell-derived neural constructs for predictive neurotoxicity screening |
|
| Relations |
| BioSample |
SAMN03254791 |
| SRA |
SRX798894 |
