|
Status |
Public on Sep 08, 2015 |
Title |
Day 21 t33b |
Sample type |
SRA |
|
|
Source name |
H1 human embryonic stem cell derived neural progenitor cells, endothelial cells, mesenchymal stem cells, and microglia/macrophage precursors
|
Organism |
Homo sapiens |
Characteristics |
screening chemicals: BMAA
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen Rneasy mini kit with RLT lysis buffer TruSeq and custom library prep
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
Human neural progenitor cells were cultured on PEG hydrogel in N2B27F2 media, endothelial cells and mesenchymal cells were then seeded on top of the NPC on day 9. Microglia/macrophage progenitors were seeded on day 13. Chemicals exposure started on day 14. Cells were lysed for RNA purification on day 21. 140304_multiplexing_Lanes123456_d7t33b
|
Data processing |
Basecalls performed using CASAVA version 1.8.2 Gene expression estimates obtained using RSEM v 1.2.3, with Bowtie 0.12.9 for alignment. Genome Reference: hg19 Supplementary_files_format_and_content: RSEM TPMs (Gene expression levels)
|
|
|
Submission date |
Dec 06, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Peng Jiang |
E-mail(s) |
p.jiang@csuohio.edu
|
Organization name |
Cleveland State University
|
Street address |
2121 Euclid Ave
|
City |
Cleveland |
State/province |
OH |
ZIP/Postal code |
44115 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE63935 |
Human pluripotent stem cell-derived neural constructs for predictive neurotoxicity screening |
|
Relations |
BioSample |
SAMN03254819 |
SRA |
SRX798964 |