|
| Status |
Public on Apr 08, 2015 |
| Title |
Total RNA, hcmv-miR-UL22A-5 siRNA |
| Sample type |
RNA |
| |
|
| Source name |
MRC-5 ATCC CCL-171
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MRC-5
tissue: lung
cell type: fibroblast
gender: male
age: 14 weeks gestation
|
| Treatment protocol |
Reverse transfection with 50 nM of hcmv-miR-UL22A-5p miScript mimic or AllStars Negative Control siRNA (Qiagen) using Lipofectamine RNAiMAX in serum-free medium (in Transfecting Stealth RNAi or siRNA into NIH 3T3 Cells Using Lipofectamine RNAiMAX, Invitrogen, Part no. 25-0906W, Rev. Date: 27Jan2006)
|
| Growth protocol |
MRC-5 cells grown for 48 hours post-transfection in EMEM 10% FBS at 37 degrees Celsius in humidified 5% CO2 atmosphere
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Manual RNA extraction utilizing miRNeasy mini kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by Alberta Transplant Applied Genomics Centre (see atagc.med.ualberta.ca)
|
| |
|
| Hybridization protocol |
Hybridization was performed by Alberta Transplant Applied Genomics Centre (see atagc.med.ualberta.ca)
|
| Scan protocol |
Scan protocol was determined by Alberta Transplant Applied Genomics Centre (see atagc.med.ualberta.ca)
|
| Description |
MRC-5 transfected with hcmv-miR-UL22A-5p from which total RNA was harvested
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package
|
| |
|
| Submission date |
Dec 24, 2014 |
| Last update date |
Apr 08, 2015 |
| Contact name |
Luiz Filipe Lisboa |
| Organization name |
University of Alberta
|
| Department |
Medical Microbiology and Immunology
|
| Street address |
6-020 Katz bldg
|
| City |
Edmonton |
| State/province |
AB |
| ZIP/Postal code |
T6G 2E1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL8971 |
| Series (1) |
| GSE64503 |
RNA-Induced Silencing Complex-associated RNA in MRC-5 cells transfected with hcmv-miR-UL22A-5p |
|
