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Status |
Public on May 27, 2016 |
Title |
H3K9me3 ChIP-Seq analysis of breast fibroblast from RM070 (A21649) |
Sample type |
SRA |
|
|
Source name |
A21649-1
|
Organism |
Homo sapiens |
Characteristics |
submitted sample id: JOC206-DNA donor_id: RM070 Sex: female body site: Breast histological type: Fibroblast cultured cell is tumor: No biomaterial_type: primary cell culture cell type: breast fibroblast
|
Extracted molecule |
genomic DNA |
Extraction protocol |
library construction protocol: Refer to document 'Chromatin Immunoprecipitation (ChIP)' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/)
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|
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Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
design description: H3K9me3 ChIP-Seq analysis of breast fibroblast from RM070 (A21649) using Illumina HiSeq 2000 library name: A21649 EXPERIMENT_TYPE: Histone H3K9me3 EXTRACTION_PROTOCOL: Refer to document 'Chromatin Immunoprecipitation (ChIP)' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/) EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Fisher 550 Sonic Dismembrator EXTRACTION_PROTOCOL_SONICATION_CYCLES: 10 min CHIP_PROTOCOL: Refer to document 'Chromatin Immunoprecipitation (ChIP)' from BC at the Roadmap Epigenomics Project site, Experimental Protocols page (URL: http://www.roadmapepigenomics.org/protocols/type/experimental/) CHIP_PROTOCOL_CHROMATIN_AMOUNT: 5 ug SIZE_FRACTION: 77-362 bp CHIP_PROTOCOL_BEAD_TYPE: Sepharose A/G Bead Mix CHIP_PROTOCOL_BEAD_AMOUNT: 20 ul CHIP_PROTOCOL_ANTIBODY_AMOUNT: 5 ug CHIP_ANTIBODY: H3K9me3 CHIP_ANTIBODY_PROVIDER: Diagenode CHIP_ANTIBODY_CATALOG: pAb-056-050 CHIP_ANTIBODY_LOT: A93-0042 **************** For data usage terms and conditions, please refer to: http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies ****************
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Data processing |
Various levels of processed data files will be made available as this project proceeds.
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|
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Submission date |
Jan 12, 2015 |
Last update date |
May 15, 2019 |
Contact name |
UCSF-UBC CENTER |
Organization name |
UCSF-UBC
|
Street address |
UCSF-UBC
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94143 |
Country |
USA |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE16368 |
UCSF-UBC Human Reference Epigenome Mapping Project |
|
Relations |
SRA |
SRX1157733 |
BioSample |
SAMN03416838 |