|
| Status |
Public on Oct 09, 2015 |
| Title |
Trisomy 21, V3036 |
| Sample type |
RNA |
| |
|
| Source name |
cultured fibroblasts, trisomy 21
|
| Organism |
Homo sapiens |
| Characteristics |
genotype/variation: trisomy 21
cell type: chorionic villi (fibroblasts)
Sex: female
gestational age (weeks of gestation): 12
|
| Treatment protocol |
After removal of the medium, trypsin EDTA was added and left to incubate for 15 minutes at 37°C. The cells were harvested, centrifuged at 1000 rpm for 10 minutes, washed with PBS and centrifuged again. The PBS was removed and the cells were lysed directly by addition of RA1 lysis buffer from a Nucleospin RNA II kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany).
|
| Growth protocol |
Standard protocols for culture of amnion cells and chorionic villi cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from each cell culture using a Nucleospin RNA II kit, according to manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from total RNA (following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA were hybridized for 16 hr at 45C on Affymetrix Human Gene 2.0 ST Array and were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix).
|
| Description |
V3036
Gene expression data.
|
| Data processing |
The data were preprocessed and analyzed using Partek Genomic Suite.
|
| |
|
| Submission date |
Jan 16, 2015 |
| Last update date |
Oct 10, 2015 |
| Contact name |
Florent Dumont |
| E-mail(s) |
florent.dumont@u-psud.fr
|
| Organization name |
Université Paris-Saclay
|
| Street address |
17 avenue des sciences
|
| City |
91400 Orsay |
| State/province |
Ile de France |
| ZIP/Postal code |
91400 |
| Country |
France |
| |
|
| Platform ID |
GPL17930 |
| Series (1) |
| GSE65055 |
Expression data from euploid and trisomic 13, 18 and 21 human fetal cells |
|
