|
| Status |
Public on Jan 01, 2017 |
| Title |
Hns – 420 min |
| Sample type |
SRA |
| |
|
| Source name |
Escherichia coli CSH50 Δhns
|
| Organism |
Escherichia coli |
| Characteristics |
strain: CSH50
genotype: delta-hns
time: 420 minutes
|
| Growth protocol |
LB medium, 37°C, airated, constant pH 7.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction Kit
GATC
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
temporal gene expression hns mutant 420 min
sample grown in LB in a fermenter @ 37°C, constant pH 7.5 and air saturated partial oxygen pressure
|
| Data processing |
GATC software for read mapping on MG1655
verified with in-house pipeline
Gene wise natural spline interpolation of temporal data from initial time points to a dataset of 10 minutes steps (see processed data files). Expression values at the time points of the raw data are not affected by the interpolation.
gene expression sum normalized to 1 at each time point
Supplementary_files_format_and_content: expression of genes in time, rows = genes, cols = time points
|
| |
|
| Submission date |
Jan 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Patrick Sobetzko |
| E-mail(s) |
patrick.sobetzko@synmikro.uni-marburg.de
|
| Organization name |
Philipps-Universität Marburg LOEWE-Zentrum für Synthetische Mikrobiologie
|
| Department |
Department of Chromosome Biology
|
| Lab |
AG Waldminghaus
|
| Street address |
Hans-Meerwein-Straße
|
| City |
Marburg |
| ZIP/Postal code |
35043 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14548 |
| Series (1) |
| GSE65244 |
Temporal gene expression in Escherichia coli |
|
| Relations |
| BioSample |
SAMN03292479 |
| SRA |
SRX851927 |
