|
Status |
Public on Apr 24, 2015 |
Title |
P19_Day_4 |
Sample type |
SRA |
|
|
Source name |
P19 cells_induced with RA
|
Organism |
Mus musculus |
Characteristics |
cell line: P19 cell type: embryonic carcinoma cells cell line info: DSMZ ACC deposit #316 cell subtype: embryoid bodies (day 4)
|
Treatment protocol |
retinoic acid induction; 1µM
|
Growth protocol |
AlphaMEM-supplemented with glutamine
|
Extracted molecule |
total RNA |
Extraction protocol |
TRIZOL Ribodepletion followed by default Illumina RNA-Seq for HiSeq2000 (TruSeq)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
NR_CS_003
|
Data processing |
Illumina base calling, demultiplexing, and FASTQ conversion with CASAVA-1.8.2 alignment to mm9 using bowtie2 20nt anchors from both ends of unaligned reads aligned to mm9 independently aligned anchors were extended to find circRNA head-to-tail splice sites (see Suppl. Methods) Genome_build: mm9 Supplementary_files_format_and_content: bed file; circRNA head-to-tail splice sites with num
|
|
|
Submission date |
Feb 13, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Petar Glazar |
Organization name |
Max Delbrück Center for Molecular Medicine
|
Department |
Systems Biology of Gene Regulatory Elements
|
Lab |
Rajewsky lab
|
Street address |
Robert Rössle Str. 10 (H. 87)
|
City |
Berlin |
ZIP/Postal code |
13092 |
Country |
Germany |
|
|
Platform ID |
GPL13112 |
Series (1) |
GSE65926 |
Circular RNAs in the mammalian brain are highly abundant, conserved, and dynamically expressed |
|
Relations |
BioSample |
SAMN03344619 |
SRA |
SRX877830 |